Sepsis Multiplex PCR Reagents with AMR markers
The Retrobiotech Sepsis Multiplex PCR Panel is a comprehensive diagnostic tool designed for the qualitative detection of bacterial and fungal pathogens associated with bloodstream infections and sepsis. This panel covers 32 key sepsis-related organisms, enabling rapid and sensitive detection of both Gram-positive and Gram-negative bacteria, as well as yeasts and opportunistic fungal pathogens. An internal control (RNAse P) is included to monitor the sample quality and PCR efficiency.
The Retrobiotech Sepsis kit consists of pre-formulated multiplex PCR reactions and standards for monitoring assay performance.
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Sepsis PCR Reaction Mix-8 Tubes Reaction Mix
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Human Internal control (HIC)
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Positive Control (pC)
| Bacteria | Fungi | AMR |
|---|---|---|
| Proteus mirabilis / Proteus vulgaris | Candida parapsilosis | Penicillin Resistance (pbp2x) |
| Escherichia coli | Candida tropicalis | Carbapenemase (KPC) |
| Streptococcus pneumoniae | Candida glabrata | Beta-lactamase (CTX-M-Grp1) |
| Klebsiella pneumoniae | Candida albicans | Beta-lactamase (SHV) |
| Streptococcus agalactiae (Group B) | Candida auris | Vancomycin Resistance (VanA) |
| Streptococcus pyogenes (Group A) | Candida krusei | Aminoglycoside Resistance (aph3) |
| Acinetobacter baumannii | Cryptococcus (C. neoformans / C. gattii) | Aminoglycoside Resistance (ant1a) |
| Enterobacter cloacae | Methicillin Resistance (mecA) | |
| Enterococcus faecalis | Tetracycline Resistance (tetM) | |
| Enterococcus faecium | Fluoroquinolone Resistance (gyrA) | |
| Pseudomonas aeruginosa | ||
| RNAseP | ||
| Staphylococcus aureus | ||
| Staphylococcus lugdunensis | ||
| Salmonella spp. | ||
| Listeria monocytogenes | ||
| Bacteroides fragilis | ||
| Neisseria meningitidis | ||
| Klebsiella oxytoca | ||
| Serratia marcescens | ||
| Staphylococcus epidermidis | ||
| Haemophilus influenzae | ||
| Klebsiella (enterobacter) aerogenes |
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Sensitivity: 10copies/ul
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Run Time: Approx 1:00 hr (depending on the instrument)
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Instrument: Applied Biosystems QuantStudio, Applied Biosystems 7500 Fast Dx (SDS software version 1.4 or later),
Bio-Rad CFX96 or CFX384 (Maestro software version 1.1 or later) real-time PCR platforms.
| Catalog# | Reactions (20µl) |
Description |
|---|---|---|
| RBT/SEP/48R | 48 | Sepsis Multiplex PCR Reagents with AMR markers. |
| RBT/Sep/96R | 96 | Sepsis Multiplex PCR Reagents with AMR markers. |
