Urinary Tract Infection (UTI) Microbial Control
Retro Biotech Urinary Tract Infection (UTI) Microbial Control (UTI-MC) is a mixture of 6 heat-inactivated organisms (two gram-positive bacteria, two gram-negative bacteria, and two fungi) known to cause UTI. UTI-MC was prepared with organisms isolated from human urine and contains intact organisms that should be processed the same as human urine specimens. UTI-MC is suitable for evaluating DNA extraction methods and molecular diagnostic assays. The extraction control pool contains the easy-to-lyse gram-negative bacteria and fungi with rigid cell walls. Therefore, UTI-MC is suitable for investigating and eliminating nucleic acid extraction bias influencing diagnostic outcomes. Additionally, UTI-MC is ideal for developing and validating molecular diagnostics tools for UTIs.
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Inactivated (heat-killed) whole organism pool.
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Microbiologically characterized organisms.
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Identity is confirmed by 16S/18S sequencing.
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NGS verified composition.
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qPCR and NGS validated Microbial composition.
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Normalized (105 genomic copies/ML) composition.
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Retrobiotech UTI-MC is suitable for evaluating qPCR and NGS assay performance for uropathogenic detection.
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Validation of clinical UTI assays and training laboratory personnel.
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Routine process control for NGS or qPCR based UTI testing
- Organisms were heat-killed to inactivate them before pooling. The inactivation was confirmed in a standard microbiological growth protocol.
- UTI-MC contains inactivated microorganisms and is not biohazardous. However, standard precautions should be followed when handling UTI-MC to prevent cross-contamination.
- For handling and disposal, consult the CDC recommendations and local regulations.
- The matrix used in this product was manufactured from artificial urine tested by qPCR and NGS and found to contain no microorganisms
- Do not use past labeled expiration date.
- To avoid cross-contamination, use sterile tips.
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Sensitivity: 10copies/ul
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Run Time: Approx 1:00 hr (depending on the instrument)
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Instrument: Applied Biosystems QuantStudio, Applied Biosystems 7500 Fast Dx (SDS software version 1.4 or later),
Bio-Rad CFX96 or CFX384 (Maestro software version 1.1 or later) real-time PCR platforms.
| Species | Class | Source | Genomic Copies/mL | PCR (Ct of 1:10) |
|---|---|---|---|---|
| Enterococcus faecalis | Gram Positive | Lab-isolate | 1.00E+05 | 27.57 |
| Enterococcus faecium | Gram Positive | Lab-isolate | 1.00E+05 | 27.81 |
| Klebsiella pneumoniae | Gram Negative | Lab-isolate | 1.00E+05 | 27.44 |
| Escherichia coli | Gram Negative | Lab-isolate | 1.00E+05 | 27.21 |
| Candida albicans | Fungi | Lab-isolate | 1.00E+05 | 26.39 |
| Candida glabrata | Fungi | Lab-isolate | 1.00E+05 | 26.08 |
