GI Multiplex PCR Reagents
Gastrointestinal Infection (GI) multiplex PCR assays are intended for the qualitative detection of GI track pathogens. The Retrobiotech GI multiplex PCR reagent kit contains multiplex PCR assays for the detection of bacterial (8), parasitic (6), viral (6) and internal control. The Retrobiotech multiplex reaction mixes (GRM-1 to GRM-8) are configured as open system reagents (OSR) for use with any real-time PCR system. Fine-tuned multiplex reactions are available in 2ml tubes or pre-plated in 384 well plates.
The Retrobiotech GI kit consists of pre-formulated multiplex PCR reactions and standards for monitoring assay performance.
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GI PCR Reaction Mix (GRM-1 to 8)
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Internal control (IC)
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Amplification Control (AC)
| Bacteria | Parasite | Virus |
|---|---|---|
| Campylobacter | Cryptosporidium spp. | Rotavirus |
| Clostridium difficile | Entamoeba histolytica | Human astrovirus |
| Enterohemorragic/verotoxin producing Escherichia coli | Enterobius vermicularis | Human adenovirus |
| Salmonella | Ancylostoma duodenale | Norovirus G1 |
| Shigella | Giardia lamblia | Norovirus G2 |
| Yersinia enterocolitica | Necator americanus | Sapovirus |
| Enteroinvasive Escherichia coli | ||
| H.Pylori RD1 |
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Sensitivity: 10copies/ul
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Run Time: Approx 1:00 hr (depending on the instrument)
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Instrument: Applied Biosystems QuantStudio, Applied Biosystems 7500 Fast Dx (SDS software version 1.4 or later),
Bio-Rad CFX96 or CFX384 (Maestro software version 1.1 or later) real-time PCR platforms.
| Catalog# | Reactions (10µl) |
Description |
|---|---|---|
| GI22-96 | 96 | GI Multiplex PCR Reagent mix - 96 Reactions |
| GI22-384 | 384 | GI Multiplex PCR Reagent mix - 384 Reactions |
| GI22- 384 PT | 48 | GI Multiplex PCR Reagents pre-plated in 384 well plate- 48 Reactions |
