Respiratory Pathogen Comprehensive Panel with AMR markers
The Retrobiotech Respiratory Pathogen Panel (RPP) is a comprehensive multiplex real-time PCR assay designed for the qualitative or semi-quantitative detection of infectious agents responsible for respiratory tract infections, along with associated antimicrobial resistance (AMR) genes. This advanced molecular diagnostic kit includes qPCR multiplex reaction mixes for detecting: 21 viral pathogens 14 bacterial pathogens 2 key antibiotic resistance markers 1 internal amplification control The RPP kit comprises 10 pre-validated multiplex reaction mixes (RRM-1 to RRM-10), configured as open system reagents (OSR) compatible with widely used real-time PCR platforms. All necessary positive and negative controls are included to ensure assay accuracy and quality assurance..
This RPP qPCR reagent kit consists of pre-formulated qPCR reactions and standards for monitoring assay performance. Scienetix reagents are available in a pre-mixed reaction mix provided in 2mL tubes. It is recommended to test amplification controls (AC) and negative controls (NC) with each lot of sample testing.
| Virus (21) | Bacteria (14) |
|---|---|
| Human coronavirus 229E | B. parapertussis |
| Human coronavirus HKU1 | Bordetella Pertussis & Holmesii |
| Human coronavirus NL63 | Chlamydophila pneumoniae |
| Human coronavirus OC43 | Klebsiella pneumoniae |
| Human gammaherpesvirus 4 (Epstin-Barr, Mono) | Moraxella catarrhalis |
| Human parechovirus | Staphylococcus aureus |
| Human bocavirus | Haemophilus influenzae |
| Human adenovirus | Haemophilus influenzae B |
| Human parainfluenza 1 virus | Salmonella typhi/paratyphi |
| Human parainfluenza 2 virus | Mycoplasma pneumoniae |
| Human parainfluenza 3 virus | Streptococcus pyogenes (GAS) |
| Human parainfluenza 4 virus | Legionella sp |
| Influenza A virus H1N1 swl | Streptococcus agalactiae (GBS) |
| Human respiratory syncytial viruses | Streptococcus pneumoniae |
| Human rhinovirus | |
| Influenza A virus | |
| Influenza B virus | AMR (2) |
| Influenza c virus | Van A/B |
| EV-A71 | Methicillin Antibiotic resistance |
| Human metapneumoviruses | |
| EV-D68 |
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Sensitivity: 10copies/ul
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Run Time: Approx 1:00 hr (depending on the instrument)
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Instrument: Applied Biosystems QuantStudio, Applied Biosystems 7500 Fast Dx (SDS software version 1.4 or later),
Bio-Rad CFX96 or CFX384 (Maestro software version 1.1 or later) real-time PCR platforms.
| Catalog# | Reactions (20µl) |
Quantity per Kit | Description |
|---|---|---|---|
| RBT/RES/35P/2AMR/48R | 48 | 1 | Respiratory Comprehensive Multiplex qPCR Reagents |
| RBT/RES/35P/2AMR/96R | 96 | 1 | Respiratory Comprehensive Multiplex qPCR Reagents |
