Retro Biotech’s Tropical Fever multiplex PCR panel is intended for the qualitative detection of pathogens associated with tropical febrile illnesses. The kit consolidates key viruses, bacteria, and a parasite target commonly implicated in outbreaks and endemic disease burden. A human internal control (IC) is included to monitor sample adequacy and PCR performance.
Reagents are manufactured and packaged as Open System Reagents (OSR) for use with Applied Biosystems QuantStudio (Design & Analysis v1.5.1+), Applied Biosystems 7500 Fast Dx (SDS v1.4+), and Bio-Rad CFX96/CFX384 (Maestro v1.1+) real-time PCR platforms. Multiplex reaction mixes (TFRM-1 to TFRM-3) are supplied as pre-mixed tubes for streamlined workflows.
The Tropical Fever kit consists of pre-formulated multiplex PCR reactions and controls for monitoring assay performance.
| Bacteria | Parasite | Virus |
|---|---|---|
| Rickettsia spp. | Plasmodium spp. (four human malaria species) | Dengue virus 1/2/3/4 |
| Salmonella spp. (typhi/paratyphi) | Chikungunya virus | |
| Brucella spp. | Zika virus | |
| Leptospira spp. | West Nile virus | |
| Japanese Encephalitis virus |
Per-mix map (TFRM-1…TFRM-3) and dye/channel assignments available on request.
| Catalog# | Reactions (20 µL) |
Description |
|---|---|---|
| RBM171-48 | 48 | Tropical Fever Multiplex PCR reagents — pre-mixed tubes (TFRM-1 to TFRM-3) |
| RBM172-96 | 96 | Tropical Fever Multiplex PCR reagents — pre-mixed tubes (TFRM-1 to TFRM-3) |
Research Use Only (RUO). Custom pack sizes available on request.