HPV Detection and Genotyping Panels

Antimicrobial Resistance Panel Sepsis Panel Meningitis/Encephalitis PCR Panel Tropical Fever PCR Panel HPV Detection & Genotyping Veterinary PCR Diagnostic Kit
Antimicrobial Resistance Panel Sepsis Panel Meningitis/Encephalitis PCR Panel Tropical Fever PCR Panel HPV Detection & Genotyping Veterinary PCR Diagnostic Kit
HPV detection and genotyping multiplex qPCR

HPV Detection and Genotyping Panels

Retro Biotech’s HPV multiplex PCR assays are intended for qualitative detection and genotyping of clinically relevant high-risk (hrHPV) and low-risk (lrHPV) Human Papillomavirus types. Targets include genotypes associated with cervical, oropharyngeal, and anogenital cancers, as well as condyloma. A human internal control (IC; RNase P) is included to verify sample integrity and PCR performance.

Reagents are manufactured and packaged as Open System Reagents (OSR) for use with Applied Biosystems QuantStudio (Design & Analysis v1.5.1+), Applied Biosystems 7500 Fast Dx (SDS v1.4+), and Bio-Rad CFX96/CFX384 (Maestro v1.1+) real-time PCR platforms. Multiplex reaction mixes (HPVRM-1 to HPVRM-4) are supplied as pre-mixed tubes for streamlined workflows.

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The HPV kit includes pre-optimized multiplex PCR mixes and essential controls for comprehensive detection and genotyping.

  • HPV PCR Reaction Mix — pre-mixed tubes (HPVRM-1 to HPVRM-4)
  • Internal Control (IC; human RNase P)
  • Positive Control (PC)
  • No-Template Control (NTC)

High-risk HPV genotypes Low-risk / Internal Controls Other Controls
HPV 16HPV 6Exogenous Extraction Control
HPV 18HPV 11Internal Control (RNase P)
HPV 31
HPV 33
HPV 35
HPV 39
HPV 45
HPV 51
HPV 52
HPV 56
HPV 58
HPV 59
HPV 66
HPV 68

Per-mix map (HPVRM-1…HPVRM-4) and dye/channel assignments available on request.

  • Sensitivity : < 10 copies/µL (per target; instrument-dependent)
  • Run Time : ~60 minutes (fast cycling)
  • Instrument : QuantStudio (D&A v1.5.1+), 7500 Fast Dx (SDS v1.4+), Bio-Rad CFX96/CFX384 (Maestro v1.1+)
  • Chemistry : Hydrolysis probe–based multiplex qPCR
  • Format : Open System Reagent (OSR)

Catalog# Reactions
(20 µL)		 Description
RBM171-48 48 HPV Detection & Genotyping multiplex PCR reagents — pre-mixed tubes (HPVRM-1 to HPVRM-4)
RBM172-96 96 HPV Detection & Genotyping multiplex PCR reagents — pre-mixed tubes (HPVRM-1 to HPVRM-4)

Research Use Only (RUO). Custom pack sizes available on request.