Sepsis Multiplex PCR Reagents with AMR Markers

Antimicrobial Resistance Panel Sepsis Panel Meningitis/Encephalitis PCR Panel Tropical Fever PCR Panel HPV Detection & Genotyping Veterinary PCR Diagnostic Kit
Antimicrobial Resistance Panel Sepsis Panel Meningitis/Encephalitis PCR Panel Tropical Fever PCR Panel HPV Detection & Genotyping Veterinary PCR Diagnostic Kit
Sepsis multiplex qPCR panel with AMR markers

Sepsis Multiplex PCR Reagents with AMR Markers

The Retro Biotech Sepsis multiplex PCR panel is intended for the qualitative detection of bacterial and fungal pathogens associated with bloodstream infections, alongside key antimicrobial resistance (AMR) markers to inform therapy. The panel covers a broad spectrum of Gram-positive and Gram-negative organisms and yeasts. A human internal control (HIC; RNase P) is included to monitor sample adequacy and PCR performance.

Reagents are manufactured and packaged as Open System Reagents (OSR) for use with Applied Biosystems QuantStudio (Design & Analysis v1.5.1+), Applied Biosystems 7500 Fast Dx (SDS v1.4+), and Bio-Rad CFX96/CFX384 (Maestro v1.1+) real-time PCR platforms.

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Safety Data Sheet

  • Sepsis PCR Reaction Mix — pre-mixed tubes (SPRM-1 to SPRM-8)
  • Human Internal Control (HIC; RNase P)
  • Positive Control (PC)
  • No-Template Control (NTC)

Bacteria Fungi AMR markers
Proteus mirabilis / P. vulgaris Candida parapsilosis Penicillin resistance (pbp2x)
Escherichia coli Candida tropicalis Carbapenemase (KPC)
Streptococcus pneumoniae Candida glabrata β-lactamase (CTX-M-Grp1)
Klebsiella pneumoniae Candida albicans β-lactamase (SHV)
Streptococcus agalactiae (Group B) Candida auris Vancomycin resistance (vanA)
Streptococcus pyogenes (Group A) Candida krusei Aminoglycoside resistance (aph3)
Acinetobacter baumannii Cryptococcus ( C. neoformans / C. gattii ) Aminoglycoside resistance (ant1a)
Enterobacter cloacae Methicillin resistance (mecA)
Enterococcus faecalis Tetracycline resistance (tetM)
Enterococcus faecium Fluoroquinolone resistance (gyrA)
Pseudomonas aeruginosa
Human RNase P (process control)
Staphylococcus aureus
Staphylococcus lugdunensis
Salmonella spp.
Listeria monocytogenes
Bacteroides fragilis
Neisseria meningitidis
Klebsiella oxytoca
Serratia marcescens
Staphylococcus epidermidis
Haemophilus influenzae
Klebsiella (Enterobacter) aerogenes

Full per-mix map (SPRM-1…SPRM-8) available on request.

  • Sensitivity : < 10 copies/µL (per target; instrument-dependent)
  • Run Time : ~60 minutes (fast cycling)
  • Instrument : QuantStudio (D&A v1.5.1+), 7500 Fast Dx (SDS v1.4+), Bio-Rad CFX96/CFX384 (Maestro v1.1+)
  • Chemistry : Hydrolysis probe–based multiplex qPCR
  • Format : Open System Reagent (OSR)

Catalog# Reactions
(20 µL)		 Description
RBM171-48 48 Sepsis Multiplex PCR Reagents with AMR markers — pre-mixed tubes (SPRM-1 to SPRM-8)
RBM172-96 96 Sepsis Multiplex PCR Reagents with AMR markers — pre-mixed tubes (SPRM-1 to SPRM-8)

Research Use Only (RUO). Custom pack sizes available on request.