Trypanosoma equiperdum Real Time PCR Detection Kit

Intended Use

The Trypanosoma equiperdum Real-Time PCR Detection Kit is designed for the detection of Trypanosoma equiperdum nucleic acid in veterinary clinical specimens collected from equids, including horses, donkeys, and mules. The kit aids in the diagnosis and monitoring of dourine, supporting early disease detection, differential diagnosis of equine trypanosomiasis, timely therapeutic intervention, disease surveillance, and effective clinical management in veterinary diagnostic laboratories.

Principle of the Assay

This kit utilizes real-time polymerase chain reaction (qPCR) technology with highly specific primers and fluorescent hydrolysis probes targeting a conserved genomic region of Trypanosoma equiperdum.

During amplification, the probe specifically hybridizes to its target sequence and generates a fluorescence signal that is detected by compatible real-time PCR instruments. The increase in fluorescence intensity is directly proportional to the accumulation of amplified product, enabling sensitive, specific, and reliable detection of Trypanosoma equiperdum nucleic acid.

Performance Characteristics

• High analytical sensitivity for early detection of Trypanosoma equiperdum
• High specificity for Trypanosoma equiperdum
• High reproducibility and assay stability
• No cross-reactivity with non-target veterinary pathogens

Sample Type

Equine EDTA-anticoagulated whole blood, buffy coat, genital swabs, vaginal swabs, urethral swabs, semen, lymph node aspirates, tissue specimens, and other appropriate veterinary clinical specimens collected from clinically suspected horses, donkeys, and mules.

Kit Features & Advantages

• Rapid and reliable molecular detection
• High sensitivity for detecting Trypanosoma equiperdum, including infections with low parasitemia
• Supports early diagnosis, differential diagnosis of equine trypanosomiasis, disease surveillance, and timely clinical intervention
• Simple workflow with minimal hands-on time
• Compatible with major real-time PCR platforms

Storage Conditions

Store at −20°C. Avoid repeated freeze-thaw cycles.