Trypanosoma brucei Real Time PCR Detection Kit

Intended Use

The Trypanosoma brucei Real-Time PCR Detection Kit is designed for the detection of Trypanosoma brucei nucleic acid in veterinary clinical specimens collected from dogs. The kit aids in the diagnosis and monitoring of trypanosome infection, supporting early disease detection, differential diagnosis of canine hemoparasitic diseases, timely therapeutic intervention, disease surveillance, and effective clinical management in veterinary diagnostic laboratories.

Principle of the Assay

This kit utilizes real-time polymerase chain reaction (qPCR) technology with highly specific primers and fluorescent hydrolysis probes targeting a conserved genomic region of Trypanosoma brucei.

During amplification, the probe specifically hybridizes to its target sequence and generates a fluorescence signal that is detected by compatible real-time PCR instruments. The increase in fluorescence intensity is directly proportional to the accumulation of amplified product, enabling sensitive, specific, and reliable detection of Trypanosoma brucei nucleic acid.

Performance Characteristics

• High analytical sensitivity for early detection of Trypanosoma brucei
• High specificity for Trypanosoma brucei
• High reproducibility and assay stability
• No cross-reactivity with non-target veterinary pathogens

Sample Type

Canine EDTA-anticoagulated whole blood, buffy coat, lymph node aspirates, bone marrow aspirates, tissue specimens, cerebrospinal fluid (when clinically indicated), and other appropriate veterinary clinical specimens collected from clinically suspected dogs.

Kit Features & Advantages

• Rapid and reliable molecular detection
• High sensitivity for detecting Trypanosoma brucei, including infections with low parasitemia
• Supports early diagnosis, differential diagnosis of canine hemoparasitic infections, and timely clinical intervention
• Simple workflow with minimal hands-on time
• Compatible with major real-time PCR platforms

Storage Conditions

Store at −20°C. Avoid repeated freeze-thaw cycles.