TickSure Canine Real Time PCR Detection Kit

Intended Use

The TickSure Canine Real-Time PCR Detection Kit is designed for the detection of nucleic acids from multiple clinically significant vector-borne pathogens associated with tick- and mosquito-borne infections in dogs. The kit aids in the diagnosis, differential identification, and monitoring of canine vector-borne diseases in veterinary clinical settings, supporting timely therapeutic intervention and effective disease management.

Principle of the Assay

This kit utilizes real-time polymerase chain reaction (PCR) technology with pathogen-specific primers and fluorescent hydrolysis probes targeting conserved genomic regions of the pathogens included in the panel. During amplification, each probe specifically hybridizes to its corresponding target sequence and generates a fluorescence signal upon cleavage by the DNA polymerase. The fluorescence is detected in real time by compatible PCR instruments, and the increase in signal is proportional to the accumulation of amplified product, enabling accurate detection and differentiation of the target pathogens. 

Performance Characteristics

• High analytical sensitivity for early detection of infection
• High analytical specificity for the targeted canine vector-borne pathogens
• Excellent reproducibility and assay stability
• No cross-reactivity with non-target canine pathogens
• Simultaneous detection of multiple vector-borne pathogens in a single assay

Sample Type

Canine EDTA-anticoagulated whole blood, buffy coat, splenic aspirates, lymph node aspirates, bone marrow aspirates, or other appropriate veterinary clinical specimens.

Kit Features & Advantages

• Rapid and accurate molecular detection with a short turnaround time
• Simultaneous detection of multiple clinically important canine vector-borne pathogens
• Supports early diagnosis and differential identification of co-infections
• Simple and easy-to-use workflow
• Compatible with major real-time PCR platforms
• Integrated canine internal control for result validation

Storage Conditions

Store at −20°C. Avoid repeated freeze–thaw cycles to maintain reagent stability and optimal assay performance.