Rinderpest virus Real Time

Intended Use

The Rinderpest Virus Real-Time PCR Detection Kit is designed for the detection of Rinderpest virus nucleic acid in veterinary clinical specimens collected from susceptible livestock. The kit aids in the diagnosis and monitoring of rinderpest virus infection, supporting early disease detection, differential diagnosis of highly contagious vesicular and febrile diseases, outbreak investigation, disease surveillance, and effective veterinary disease control in diagnostic laboratories.

Principle of the Assay

This kit utilizes real-time polymerase chain reaction (qPCR) technology with highly specific primers and fluorescent hydrolysis probes targeting a conserved genomic region of the Rinderpest virus genome.

During amplification, the probe specifically hybridizes to its target sequence and generates a fluorescence signal that is detected by compatible real-time PCR instruments. The increase in fluorescence intensity is directly proportional to the accumulation of amplified product, enabling sensitive, specific, and reliable detection of Rinderpest virus nucleic acid.

Performance Characteristics

• High analytical sensitivity for early detection of Rinderpest virus
• High specificity for Rinderpest virus
• High reproducibility and assay stability
• No cross-reactivity with non-target veterinary pathogens

Sample Type

Bovine and other susceptible livestock EDTA-anticoagulated whole blood, ocular swabs, nasal swabs, oral swabs, lymph node aspirates, spleen, lung and intestinal tissues, post-mortem tissues, and other appropriate veterinary clinical specimens collected from clinically suspected animals.

Kit Features & Advantages

• Rapid and reliable molecular detection
• High sensitivity for detecting Rinderpest virus, including during the early stages of infection
• Supports early diagnosis, outbreak investigation, disease surveillance, and timely implementation of control measures
• Simple workflow with minimal hands-on time
• Compatible with major real-time PCR platforms

Storage Conditions

Store at −20°C. Avoid repeated freeze-thaw cycles.