Rabies Virus Real Time PCR Detection Kit

Intended Use

The Rabies Virus RT-PCR Detection Kit is designed for the detection of Rabies virus RNA in veterinary clinical specimens collected from susceptible animals. The kit aids in the diagnosis and confirmation of rabies virus infection, supporting early disease detection, differential diagnosis of neurological diseases, timely implementation of infection control and public health measures, disease surveillance, and effective clinical management in veterinary diagnostic laboratories.

Principle of the Assay

This kit utilizes reverse transcription real-time polymerase chain reaction (RT-qPCR) technology with highly specific primers and fluorescent hydrolysis probes targeting a conserved genomic region of the Rabies virus genome.

During the reverse transcription step, viral RNA is converted into complementary DNA (cDNA), which is subsequently amplified by real-time PCR. The probe specifically hybridizes to its target sequence and generates a fluorescence signal that is detected by compatible real-time PCR instruments. The increase in fluorescence intensity is directly proportional to the accumulation of amplified product, enabling sensitive, specific, and reliable detection of Rabies virus RNA.

Performance Characteristics

• High analytical sensitivity for early detection of Rabies virus
• High specificity for Rabies virus
• High reproducibility and assay stability
• No cross-reactivity with non-target veterinary pathogens

Sample Type

Brain tissue (including hippocampus, cerebellum, and brainstem), saliva, oral swabs, cerebrospinal fluid (CSF), nuchal skin biopsies, salivary gland tissue, post-mortem brain specimens, and other appropriate veterinary clinical specimens collected from clinically suspected animals.

Kit Features & Advantages

• Rapid and reliable molecular detection
• High sensitivity for detecting Rabies virus, including samples with low viral RNA levels
• Supports early diagnosis, differential diagnosis of neurological diseases, disease surveillance, and timely implementation of rabies control measures
• Simple workflow with minimal hands-on time
• Compatible with major real-time PCR platforms

Storage Conditions

Store at −20°C. Avoid repeated freeze-thaw cycles.