Plasmodium species Real Time PCR Detection KIt

Intended Use

The Plasmodium Species Real Time PCR Detection Kit is designed for the detection of Plasmodium species nucleic acid in clinical specimens. The kit aids in the diagnosis and monitoring of malaria, supporting early disease detection, differential diagnosis of Plasmodium infections, timely therapeutic intervention, treatment monitoring, and disease surveillance in clinical and public health laboratories.

Principle of the Assay

This kit utilizes real-time polymerase chain reaction (qPCR) technology with highly specific primers and fluorescent hydrolysis probes targeting a conserved genomic region of Plasmodium species.

During amplification, the probe specifically hybridizes to its target sequence and generates a fluorescence signal that is detected by compatible real-time PCR instruments. The increase in fluorescence intensity is directly proportional to the accumulation of amplified product, enabling sensitive, specific, and reliable detection of Plasmodium species nucleic acid.

Performance Characteristics

• High analytical sensitivity for early detection of Plasmodium species
• High specificity for Plasmodium species
• High reproducibility and assay stability
• No cross-reactivity with non-target pathogens

Sample Type

Human EDTA-anticoagulated whole blood, capillary blood, buffy coat, dried blood spots (DBS), and other appropriate clinical specimens collected from individuals with suspected malaria.

Kit Features & Advantages

• Rapid and reliable molecular detection
• High sensitivity for detecting Plasmodium species, including low-parasitemia infections
• Supports early diagnosis, differential detection of malaria infections, treatment monitoring, and disease surveillance
• Simple workflow with minimal hands-on time
• Compatible with major real-time PCR platforms

Storage Conditions

Store at −20°C. Avoid repeated freeze-thaw cycles.