Pan Foot-and-Mouth Disease Virus Real Time PCR Detection Kit

Intended Use

The Pan Foot-and-Mouth Disease Virus Screening Kit is designed for the detection of Foot-and-Mouth Disease Virus (FMDV) nucleic acid in veterinary clinical specimens collected from susceptible cloven-hoofed animals. The kit aids in the diagnosis and screening of foot-and-mouth disease, supporting early disease detection, differential diagnosis of vesicular diseases, rapid outbreak investigation, disease surveillance, and effective veterinary disease control in diagnostic laboratories.

Principle of the Assay

This kit utilizes real-time polymerase chain reaction (qPCR) technology with highly specific primers and fluorescent hydrolysis probes targeting a highly conserved genomic region of the Foot-and-Mouth Disease Virus genome, enabling broad detection of all major FMDV serotypes.

During amplification, the probe specifically hybridizes to its target sequence and generates a fluorescence signal that is detected by compatible real-time PCR instruments. The increase in fluorescence intensity is directly proportional to the accumulation of amplified product, enabling sensitive, specific, and reliable detection of Foot-and-Mouth Disease Virus nucleic acid.

Performance Characteristics

• High analytical sensitivity for early detection of Foot-and-Mouth Disease Virus
• Broad detection of all major FMDV serotypes
• High reproducibility and assay stability
• No cross-reactivity with non-target veterinary pathogens

Sample Type

Vesicular fluid, epithelial tissue from vesicular lesions, vesicular swabs, oral swabs, nasal swabs, probang (oropharyngeal) samples, EDTA-anticoagulated whole blood, milk, tissue specimens, post-mortem tissues, and other appropriate veterinary clinical specimens collected from clinically suspected susceptible livestock.

Kit Features & Advantages

• Rapid and reliable molecular detection
• Broad screening capability for all major Foot-and-Mouth Disease Virus serotypes
• Supports early diagnosis, differential diagnosis of vesicular diseases, outbreak investigation, and disease surveillance
• Simple workflow with minimal hands-on time
• Compatible with major real-time PCR platforms

Storage Conditions

Store at −20°C. Avoid repeated freeze-thaw cycles.