Lumpy Skin Disease Virus Real Time PCR Detection Kit

Intended Use

The Lumpy Skin Disease Virus Real-Time PCR Detection Kit is designed for the detection of Lumpy Skin Disease Virus (LSDV) nucleic acid in veterinary clinical specimens collected from cattle. The kit aids in the diagnosis and monitoring of lumpy skin disease, supporting early disease detection, differential diagnosis of nodular and vesicular skin diseases, timely implementation of outbreak control measures, disease surveillance, and effective clinical management in veterinary diagnostic laboratories.

Principle of the Assay

This kit utilizes real-time polymerase chain reaction (qPCR) technology with highly specific primers and fluorescent hydrolysis probes targeting a conserved genomic region of the Lumpy Skin Disease Virus genome.

During amplification, the probe specifically hybridizes to its target sequence and generates a fluorescence signal that is detected by compatible real-time PCR instruments. The increase in fluorescence intensity is directly proportional to the accumulation of amplified product, enabling sensitive, specific, and reliable detection of Lumpy Skin Disease Virus nucleic acid.

Performance Characteristics

• High analytical sensitivity for early detection of Lumpy Skin Disease Virus
• High specificity for Lumpy Skin Disease Virus
• High reproducibility and assay stability
• No cross-reactivity with non-target veterinary pathogens

Sample Type

Bovine skin nodules, scab material, skin biopsies, EDTA-anticoagulated whole blood, serum, plasma, nasal swabs, ocular swabs, lymph node aspirates, tissue specimens, post-mortem tissues, and other appropriate veterinary clinical specimens collected from clinically suspected cattle.

Kit Features & Advantages

• Rapid and reliable molecular detection
• High sensitivity for detecting Lumpy Skin Disease Virus, including during the early stages of infection
• Supports early diagnosis, differential diagnosis of nodular skin diseases, outbreak investigation, and timely implementation of disease control measures
• Simple workflow with minimal hands-on time
• Compatible with major real-time PCR platforms

Storage Conditions

Store at −20°C. Avoid repeated freeze-thaw cycles.