Infectious Bovine Rhinotracheitis (BoHV-1) Real Time PCR Detection Kit

Intended Use

The Infectious Bovine Rhinotracheitis (BoHV-1) Real-Time PCR Detection Kit is designed for the detection of Bovine Herpesvirus 1 (BoHV-1) nucleic acid in veterinary clinical specimens. The kit aids in the diagnosis and monitoring of bovine herpesviral infection, supporting early disease detection, timely therapeutic intervention, implementation of appropriate infection control measures, and effective clinical management in veterinary diagnostic laboratories.

Principle of the Assay

This kit utilizes real-time polymerase chain reaction (qPCR) technology with highly specific primers and fluorescent hydrolysis probes targeting a conserved genomic region of Bovine Herpesvirus 1.

During amplification, the probe specifically hybridizes to the target sequence and generates a fluorescence signal that is detected by compatible real-time PCR instruments. The increase in fluorescence intensity is directly proportional to the accumulation of amplified product, enabling sensitive, specific, and reliable detection of Bovine Herpesvirus 1 nucleic acid.

Performance Characteristics

• High analytical sensitivity for early detection of Bovine Herpesvirus 1
• High specificity for Bovine Herpesvirus 1
• High reproducibility and assay stability
• No cross-reactivity with non-target veterinary pathogens

Sample Type

Bovine nasal swabs, conjunctival swabs, respiratory secretions, EDTA-anticoagulated whole blood, genital secretions, semen, aborted fetal tissue, and other appropriate veterinary clinical specimens collected from clinically suspected cattle.

Kit Features & Advantages

• Rapid and reliable molecular detection
• High sensitivity for detecting Bovine Herpesvirus 1, including during the early stage of infection
• Supports early diagnosis and prompt clinical intervention
• Simple workflow with minimal hands-on time
• Compatible with major real-time PCR platforms

Storage Conditions

Store at −20°C. Avoid repeated freeze-thaw cycles.