Hemoprotozoan Panel - 1 Real Time PCR Detection Kit

Intended Use

The Hemaprotozoan Panel-1 Real-Time PCR Detection Kit is designed for the detection of nucleic acids from multiple tick-borne hemoparasitic pathogens associated with canine vector-borne diseases in dogs. The kit aids in the diagnosis, differential identification, and monitoring of hemoprotozoan and rickettsial infections in veterinary clinical settings.

Principle of the Assay

This kit utilizes real-time polymerase chain reaction (qPCR) technology with specific primers and fluorescent probes targeting conserved genomic regions of the pathogens included in the panel.

During amplification, each probe hybridizes to its corresponding target sequence and emits fluorescence, which is detected by compatible real-time PCR instruments. The increase in fluorescence signal is proportional to the accumulation of amplified product, enabling accurate detection of the pathogens present in the sample. A canine internal control is incorporated to monitor nucleic acid extraction efficiency, amplification performance, and assay integrity.

Performance Characteristics

• High analytical sensitivity for early-stage infection
• High specificity for included tick-borne pathogens
• High reproducibility and stability
• No cross-reactivity with unrelated canine pathogens

Sample Type

• EDTA-anticoagulated canine whole blood (Buffy coat may also be used)

Kit Features & Advantages

• Rapid detection with short turnaround time
• Simultaneous detection of multiple tick-borne pathogens
• Supports identification of co-infections
• Easy-to-operate workflow
• Compatible with major real-time PCR platforms

Storage Conditions

• Store at -20°C.
• Avoid repeated freeze-thaw cycles.