Francisella tularensis Real Time PCR Detection Kit

Intended Use

The Francisella tularensis Real-Time PCR Detection Kit is designed for the detection of Francisella tularensis nucleic acid in veterinary clinical specimens collected from cats, dogs, rabbits, hares, rodents, sheep, and other susceptible animal species. The kit aids in the diagnosis and monitoring of tularemia, supporting early disease detection, differential diagnosis of bacterial infections, timely therapeutic intervention, disease surveillance, and effective clinical management in veterinary diagnostic laboratories.

Principle of the Assay

This kit utilizes real-time polymerase chain reaction (qPCR) technology with highly specific primers and fluorescent hydrolysis probes targeting a conserved genomic region of Francisella tularensis.

During amplification, the probe specifically hybridizes to its target sequence and generates a fluorescence signal that is detected by compatible real-time PCR instruments. The increase in fluorescence intensity is directly proportional to the accumulation of amplified product, enabling sensitive, specific, and reliable detection of Francisella tularensis nucleic acid.

Performance Characteristics

• High analytical sensitivity for early detection of Francisella tularensis
• High specificity for Francisella tularensis
• High reproducibility and assay stability
• No cross-reactivity with non-target veterinary pathogens

Sample Type

EDTA-anticoagulated whole blood, lymph node aspirates, spleen aspirates, liver tissue, lung tissue, ulcer or lesion swabs, tissue specimens, and other appropriate veterinary clinical specimens collected from clinically suspected cats, dogs, rabbits, hares, rodents, sheep, and other susceptible animal species.

Kit Features & Advantages

• Rapid and reliable molecular detection
• High sensitivity for detecting Francisella tularensis, including during the early stage of infection
• Supports early diagnosis, differential diagnosis of bacterial infections, and timely clinical intervention
• Simple workflow with minimal hands-on time
• Compatible with major real-time PCR platforms

Storage Conditions

Store at −20°C. Avoid repeated freeze-thaw cycles.