Equine herpesvirus 4 (EHV-4) Real Time PCR Detection Kit

Intended Use

The Equine Herpesvirus 4 (EHV-4) Real-Time PCR Detection Kit is designed for the detection of Equine Herpesvirus 4 (EHV-4) nucleic acid in veterinary clinical specimens collected primarily from horses. The kit aids in the diagnosis and monitoring of EHV-4 infection, supporting early disease detection, differential diagnosis of equine respiratory diseases, timely implementation of biosecurity measures, disease surveillance, and effective clinical management in veterinary diagnostic laboratories.

Principle of the Assay

This kit utilizes real-time polymerase chain reaction (qPCR) technology with highly specific primers and fluorescent hydrolysis probes targeting a conserved genomic region of the Equine Herpesvirus 4 (EHV-4) genome.

During amplification, the probe specifically hybridizes to its target sequence and generates a fluorescence signal that is detected by compatible real-time PCR instruments. The increase in fluorescence intensity is directly proportional to the accumulation of amplified product, enabling sensitive, specific, and reliable detection of Equine Herpesvirus 4 (EHV-4) nucleic acid.

Performance Characteristics

• High analytical sensitivity for early detection of Equine Herpesvirus 4 (EHV-4)
• High specificity for Equine Herpesvirus 4 (EHV-4)
• High reproducibility and assay stability
• No cross-reactivity with non-target veterinary pathogens

Sample Type

Equine nasal swabs, nasopharyngeal swabs, EDTA-anticoagulated whole blood, buffy coat, tracheal washes, bronchoalveolar lavage (BAL) fluid, lymph node aspirates, lung tissue, and other appropriate veterinary clinical specimens collected from clinically suspected horses.

Kit Features & Advantages

• Rapid and reliable molecular detection
• High sensitivity for detecting Equine Herpesvirus 4 (EHV-4), including during the early stages of infection
• Supports early diagnosis, differential diagnosis of equine respiratory diseases, disease surveillance, and timely implementation of biosecurity measures
• Simple workflow with minimal hands-on time
• Compatible with major real-time PCR platforms

Storage Conditions

Store at −20°C. Avoid repeated freeze-thaw cycles.