Cytauxzoon Felis Real Time PCR Detection Kit

Intended Use

The Cytauxzoon felis Real-Time PCR Detection Kit is designed for the detection of Cytauxzoon felis nucleic acid in veterinary clinical specimens collected from cats. The kit aids in the diagnosis and monitoring of cytauxzoonosis, supporting early disease detection, differential diagnosis of feline hemoparasitic infections, timely therapeutic intervention, disease surveillance, and effective clinical management in veterinary diagnostic laboratories.

Principle of the Assay

This kit utilizes real-time polymerase chain reaction (qPCR) technology with highly specific primers and fluorescent hydrolysis probes targeting a conserved genomic region of Cytauxzoon felis.

During amplification, the probe specifically hybridizes to its target sequence and generates a fluorescence signal that is detected by compatible real-time PCR instruments. The increase in fluorescence intensity is directly proportional to the accumulation of amplified product, enabling sensitive, specific, and reliable detection of Cytauxzoon felis nucleic acid.

Performance Characteristics

• High analytical sensitivity for early detection of Cytauxzoon felis
• High specificity for Cytauxzoon felis
• High reproducibility and assay stability
• No cross-reactivity with non-target veterinary pathogens

Sample Type

Feline EDTA-anticoagulated whole blood, buffy coat, bone marrow aspirates, spleen aspirates, tissue specimens, lymph node aspirates, and other appropriate veterinary clinical specimens collected from clinically suspected cats.

Kit Features & Advantages

• Rapid and reliable molecular detection
• High sensitivity for detecting Cytauxzoon felis, including infections with low parasitemia
• Supports early diagnosis, differential diagnosis of feline hemoparasitic infections, and timely clinical intervention
• Simple workflow with minimal hands-on time
• Compatible with major real-time PCR platforms

Storage Conditions

Store at −20°C. Avoid repeated freeze-thaw cycles.