Canine B-H Real Time PCR Detection Kit

Intended Use

The Canine B-H Real-Time PCR Detection Kit is designed for the detection of nucleic acids from Babesia gibsoni, Babesia canis, and Hepatozoon canis in canine clinical specimens. The kit aids in the diagnosis, differentiation, and monitoring of canine vector-borne protozoal infections, supporting timely therapeutic intervention, disease management, and epidemiological surveillance in veterinary diagnostic laboratories and clinical settings.

Principle of the Assay

This kit utilizes real-time polymerase chain reaction (qPCR) technology with highly specific primers and fluorescent hydrolysis probes targeting conserved genomic regions of Babesia gibsoni, Babesia canis, and Hepatozoon canis.

During amplification, each probe specifically hybridizes to its corresponding target sequence and generates a fluorescence signal that is detected by compatible real-time PCR instruments. The increase in fluorescence intensity is directly proportional to the accumulation of amplified product, enabling sensitive, specific, and simultaneous detection and differentiation of the targeted protozoan pathogens.

Performance Characteristics

• High analytical sensitivity for early detection of infection
• High specificity for Babesia gibsoni, Babesia canis, and Hepatozoon canis
• High reproducibility and assay stability
• No cross-reactivity with non-target canine pathogens

Sample Type

Canine EDTA-anticoagulated whole blood, buffy coat, spleen aspirates, bone marrow aspirates, lymph node aspirates, and other appropriate veterinary clinical specimens collected from clinically suspected dogs.

Kit Features & Advantages

• Rapid and reliable molecular detection
• Simultaneous detection and differentiation of multiple canine protozoan pathogens
• Supports early diagnosis and appropriate treatment decisions
• Simple workflow with minimal hands-on time
• Compatible with major real-time PCR platforms

Storage Conditions

Store at -20°C. Avoid repeated freeze-thaw cycles