Burkholderia mallei Real Time PCR Detection Kit

Intended Use

The Burkholderia mallei Real-Time PCR Detection Kit is designed for the detection of Burkholderia mallei nucleic acid in veterinary clinical specimens collected from equids, including horses, donkeys, and mules. The kit aids in the diagnosis and monitoring of glanders, supporting early disease detection, differential diagnosis of equine bacterial infections, timely implementation of disease control measures, outbreak investigation, disease surveillance, and effective clinical management in veterinary diagnostic laboratories.

Principle of the Assay

This kit utilizes real-time polymerase chain reaction (qPCR) technology with highly specific primers and fluorescent hydrolysis probes targeting a conserved genomic region of Burkholderia mallei.

During amplification, the probe specifically hybridizes to its target sequence and generates a fluorescence signal that is detected by compatible real-time PCR instruments. The increase in fluorescence intensity is directly proportional to the accumulation of amplified product, enabling sensitive, specific, and reliable detection of Burkholderia mallei nucleic acid.

Performance Characteristics

• High analytical sensitivity for early detection of Burkholderia mallei
• High specificity for Burkholderia mallei
• High reproducibility and assay stability
• No cross-reactivity with non-target veterinary pathogens

Sample Type

Equine nasal swabs, nasal exudates, pus from cutaneous lesions, lymph node aspirates, EDTA-anticoagulated whole blood, tissue specimens (including lung, liver, and spleen), tracheal aspirates, post-mortem tissues, and other appropriate veterinary clinical specimens collected from clinically suspected horses, donkeys, and mules.

Kit Features & Advantages

• Rapid and reliable molecular detection
• High sensitivity for detecting Burkholderia mallei, including early-stage infections
• Supports early diagnosis, differential diagnosis of equine bacterial diseases, outbreak investigation, disease surveillance, and timely implementation of control measures
• Simple workflow with minimal hands-on time
• Compatible with major real-time PCR platforms

Storage Conditions

Store at −20°C. Avoid repeated freeze-thaw cycles.