Bovine Circovirus Real Time PCR Detection KIT

Intended Use

The Bovine Circovirus Real-Time PCR Detection Kit is designed for the detection of Bovine Circovirus (BoCV) nucleic acid in veterinary clinical specimens collected from cattle. The kit aids in the diagnosis and monitoring of bovine circovirus infection, supporting early disease detection, differential diagnosis of respiratory, enteric, and systemic infections, timely herd health management, disease surveillance, and effective clinical management in veterinary diagnostic laboratories.

Principle of the Assay

This kit utilizes real-time polymerase chain reaction (qPCR) technology with highly specific primers and fluorescent hydrolysis probes targeting a conserved genomic region of the Bovine Circovirus genome.

During amplification, the probe specifically hybridizes to its target sequence and generates a fluorescence signal that is detected by compatible real-time PCR instruments. The increase in fluorescence intensity is directly proportional to the accumulation of amplified product, enabling sensitive, specific, and reliable detection of Bovine Circovirus nucleic acid.

Performance Characteristics

• High analytical sensitivity for early detection of Bovine Circovirus
• High specificity for Bovine Circovirus
• High reproducibility and assay stability
• No cross-reactivity with non-target veterinary pathogens

Sample Type

Bovine EDTA-anticoagulated whole blood, serum, plasma, nasal swabs, fecal specimens, lymph node aspirates, spleen, lung, intestinal tissues, post-mortem tissue specimens, and other appropriate veterinary clinical specimens collected from clinically suspected cattle.

Kit Features & Advantages

• Rapid and reliable molecular detection
• High sensitivity for detecting Bovine Circovirus, including infections with low viral loads
• Supports early diagnosis, differential diagnosis of respiratory, enteric, and systemic infections, and timely herd health management
• Simple workflow with minimal hands-on time
• Compatible with major real-time PCR platforms

Storage Conditions

Store at −20°C. Avoid repeated freeze-thaw cycles.