Intended Use
The Babesia microti Real-Time PCR Detection Kit is designed for the detection of Babesia microti nucleic acid in veterinary clinical specimens collected from dogs and other susceptible animal species. The kit aids in the diagnosis and monitoring of babesial infection, supporting early disease detection, differential diagnosis of hemoparasitic diseases, timely therapeutic intervention, disease surveillance, and effective clinical management in veterinary diagnostic laboratories.
Principle of the Assay
This kit utilizes real-time polymerase chain reaction (qPCR) technology with highly specific primers and fluorescent hydrolysis probes targeting a conserved genomic region of Babesia microti.
During amplification, the probe specifically hybridizes to its target sequence and generates a fluorescence signal that is detected by compatible real-time PCR instruments. The increase in fluorescence intensity is directly proportional to the accumulation of amplified product, enabling sensitive, specific, and reliable detection of Babesia microti nucleic acid.
Performance Characteristics
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High analytical sensitivity for early detection of Babesia microti
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High specificity for Babesia microti
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High reproducibility and assay stability
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No cross-reactivity with non-target veterinary pathogens
Sample Type
EDTA-anticoagulated whole blood, buffy coat, bone marrow aspirates, spleen aspirates, tissue specimens, and other appropriate veterinary clinical specimens collected from clinically suspected dogs and other susceptible animal species.
Kit Features & Advantages
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Rapid and reliable molecular detection
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High sensitivity for detecting Babesia microti, including infections with low parasitemia
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Supports early diagnosis, differential diagnosis of hemoparasitic infections, and timely clinical intervention
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Simple workflow with minimal hands-on time
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Compatible with major real-time PCR platforms
Storage Conditions
Store at −20°C. Avoid repeated freeze-thaw cycles.
Anti Microbial Resistance Marker
