Babesia caballi Real Time PCR Detection Kit

Intended Use

The Babesia caballi Real-Time PCR Detection Kit is designed for the detection of Babesia caballi nucleic acid in veterinary clinical specimens collected primarily from horses. The kit aids in the diagnosis and monitoring of equine piroplasmosis, supporting early disease detection, differential diagnosis of equine hemoparasitic diseases, timely therapeutic intervention, disease surveillance, and effective clinical management in veterinary diagnostic laboratories.

Principle of the Assay

This kit utilizes real-time polymerase chain reaction (qPCR) technology with highly specific primers and fluorescent hydrolysis probes targeting a conserved genomic region of Babesia caballi.

During amplification, the probe specifically hybridizes to its target sequence and generates a fluorescence signal that is detected by compatible real-time PCR instruments. The increase in fluorescence intensity is directly proportional to the accumulation of amplified product, enabling sensitive, specific, and reliable detection of Babesia caballi nucleic acid.

Performance Characteristics

• High analytical sensitivity for early detection of Babesia caballi
• High specificity for Babesia caballi
• High reproducibility and assay stability
• No cross-reactivity with non-target veterinary pathogens

Sample Type

Equine EDTA-anticoagulated whole blood, buffy coat, bone marrow aspirates, spleen aspirates, tissue specimens, and other appropriate veterinary clinical specimens collected from clinically suspected horses.

Kit Features & Advantages

• Rapid and reliable molecular detection
• High sensitivity for detecting Babesia caballi, including infections with low parasitemia
• Supports early diagnosis, differential diagnosis of equine hemoparasitic diseases, disease surveillance, and timely clinical intervention
• Simple workflow with minimal hands-on time
• Compatible with major real-time PCR platforms

Storage Conditions

Store at −20°C. Avoid repeated freeze-thaw cycles.