Porcine vesicular Disease Panel Real-Time PCR Detection Kit

Intended Use
The Porcine Vesicular Disease Panel Real-Time PCR Detection Kit is designed for the qualitative detection of nucleic acids from multiple pathogens associated with vesicular and skin lesions in pigs. The kit aids in the diagnosis, differential identification, and monitoring of vesicular and dermatologic infections in veterinary clinical settings.

Principle of the Assay
This kit utilizes real-time polymerase chain reaction (qPCR) technology with specific primers and fluorescent probes targeting conserved genomic regions of the pathogens included in the panel.

During amplification, each probe hybridizes to its target sequence and emits fluorescence, which is detected by compatible real-time PCR instruments. The increase in fluorescence signal is proportional to the accumulation of amplified product, allowing accurate detection of the pathogens present in the sample.

Performance Characteristics

• High analytical sensitivity for early-stage infection

• High specificity for included vesicular disease pathogens

• High reproducibility and stability

• No cross-reactivity with unrelated porcine pathogens

Sample Type

• EDTA-anticoagulated whole blood, vesicular fluid, or tissue samples (porcine)

Kit Features & Advantages

• Rapid detection with short turnaround time

• Simultaneous detection of multiple vesicular and dermatologic pathogens

• Easy-to-operate workflow

• Compatible with major real-time PCR platforms

Storage Conditions

• Store at -20°C. Avoid repeated freeze-thaw cycles.