Bovine respiratory Panel

Intended Use
The Bovine Respiratory Panel Real-Time PCR Detection Kit is designed for the qualitative detection of nucleic acids from multiple bovine respiratory pathogens extracted from cattle clinical samples. The kit aids in the diagnosis and monitoring of bovine respiratory disease complex (BRDC) in veterinary clinical settings.

Principle of the Assay
This kit utilizes real-time polymerase chain reaction (qPCR) technology with specific primers and fluorescent probes targeting conserved regions of the genomes of key bovine respiratory pathogens.

During amplification, the probe hybridizes to the target sequence and emits fluorescence, which is detected by compatible real-time PCR instruments. The increase in fluorescence signal is proportional to the accumulation of amplified product, allowing accurate detection of each pathogen included in the panel.

Performance Characteristics
Sensitivity: 100%

Specificity: 100%

High reproducibility and stability

No cross-reactivity with other common bovine bacterial or viral pathogens not included in the panel

Sample Type
Nasal swabs, deep nasopharyngeal swabs, and respiratory secretions (cattle)
EDTA-anticoagulated whole blood (for systemic pathogens included in the panel)

Kit Features & Advantages
Rapid detection with short turnaround time

High analytical sensitivity for early-stage infection

Simultaneous detection of multiple bovine respiratory pathogens

Easy-to-operate workflow

Compatible with major real-time PCR platforms

Storage Conditions
Store at -20°C. Avoid repeated freeze-thaw cycles.