Intended Use
The Mycobacterium tuberculosis Complex Real-Time PCR Detection Kit is designed for the qualitative detection of Mycobacterium tuberculosis complex DNA extracted from canine and feline clinical samples. The kit aids in the diagnosis and monitoring of tuberculosis in companion animals.
Principle of the Assay
This kit utilizes real-time polymerase chain reaction (qPCR) technology with specific primers and fluorescent probes targeting conserved regions of the Mycobacterium tuberculosis complex genome.
During amplification, the probe hybridizes to the target sequence and emits fluorescence, which is detected by compatible real-time PCR instruments. The increase in fluorescence signal is proportional to the accumulation of amplified product, allowing accurate detection of Mycobacterium tuberculosis complex DNA.
Performance Characteristics
Sensitivity: 100%
Specificity: 100%
High reproducibility and stability
No cross-reactivity with other common canine and feline bacterial pathogens
Sample Type
EDTA-anticoagulated whole blood (canine and feline)
Fine-needle aspirates or tissue biopsies (suspected tuberculosis lesions)
Kit Features & Advantages
Rapid detection with short turnaround time
High analytical sensitivity for early-stage infection
Specific detection of Mycobacterium tuberculosis complex
Easy-to-operate workflow
Compatible with major real-time PCR platforms
Storage Conditions
Store at -20°C. Avoid repeated freeze-thaw cycles.
Anti Microbial Resistance Marker
