The Leptospira spp. Real-Time PCR Detection Kit is designed for the qualitative detection of Leptospira spp. DNA extracted from canine and feline clinical samples. The kit aids in the diagnosis and monitoring of leptospirosis in veterinary clinical settings.
Principle of the Assay
This kit utilizes real-time polymerase chain reaction (qPCR) technology with specific primers and fluorescent probes targeting conserved regions of the Leptospira spp. genome.
During amplification, the probe hybridizes to the target sequence and emits fluorescence, which is detected by compatible real-time PCR instruments. The increase in fluorescence signal is proportional to the accumulation of amplified product, allowing accurate detection of Leptospira spp.
Performance Characteristics
Sensitivity: 100%
Specificity: 100%
High reproducibility and stability
No cross-reactivity with other common canine and feline bacterial pathogens
Sample Type
EDTA-anticoagulated whole blood (canine and feline)
Urine samples (canine and feline)
Kit Features & Advantages
Rapid detection with short turnaround time
High analytical sensitivity for early-stage infection
Specific detection of Leptospira spp.
Easy-to-operate workflow
Compatible with major real-time PCR platforms
Storage Conditions
Store at -20°C. Avoid repeated freeze-thaw cycles.
Anti Microbial Resistance Marker
