Intended Use
The Brucella spp. Real-Time PCR Detection Kit is designed for the qualitative detection of Brucella species DNA extracted from canine and feline whole blood or tissue samples. The kit aids in the diagnosis and monitoring of brucellosis in veterinary clinical settings.
Principle of the Assay
This kit utilizes real-time polymerase chain reaction (qPCR) technology with specific primers and fluorescent probes targeting conserved regions of the Brucella genome.
During amplification, the probe hybridizes to the target sequence and emits fluorescence, which is detected by compatible real-time PCR instruments. The increase in fluorescence signal is proportional to the accumulation of amplified product, allowing accurate detection of the pathogen.
Performance Characteristics
Sensitivity: 100%
Specificity: 100%
High reproducibility and stability
No cross-reactivity with other common canine and feline blood or tissue pathogens
Sample Type
EDTA-anticoagulated whole blood, tissue, or other clinical specimens (canine and feline)
Kit Features & Advantages
Rapid detection with short turnaround time
High analytical sensitivity for early-stage infection
Specific detection of Brucella spp.
Easy-to-operate workflow
Compatible with major real-time PCR platforms
Storage Conditions
Store at -20°C. Avoid repeated freeze-thaw cycles.
Anti Microbial Resistance Marker
