Anaplasma phagocytophilum Detection kit

Intended Use

The Anaplasma phagocytophilum Real-Time PCR Detection Kit is designed for the qualitative detection of Anaplasma phagocytophilum DNA extracted from canine and feline whole blood samples. The kit aids in the diagnosis and monitoring of anaplasmosis caused by Anaplasma phagocytophilum in veterinary clinical settings.

Principle of the Assay

This kit utilizes real-time polymerase chain reaction (qPCR) technology with specific primers and fluorescent probes targeting conserved regions of the Anaplasma phagocytophilum genome.

During amplification, the probe hybridizes to the target sequence and emits fluorescence, which is detected by compatible real-time PCR instruments. The increase in fluorescence signal is proportional to the accumulation of amplified product, allowing accurate detection of the pathogen.

Performance Characteristics

• Sensitivity: 100%

• Specificity: 100%

• High reproducibility and stability

• No cross-reactivity with other common canine and feline blood-borne pathogens

Sample Type

EDTA-anticoagulated whole blood (canine and feline)

Kit Features & Advantages

• Rapid detection with short turnaround time

• High analytical sensitivity for early-stage infection

• Specific detection of Anaplasma phagocytophilum

• Easy-to-operate workflow

• Compatible with major real-time PCR platforms

Storage Conditions

Store at -20°C. Avoid repeated freeze–thaw cycles.